Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes†

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibodylike fragments (yeast-scFv) as novel affinity reagents.
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